Wood and sulfur-based cyclic denitrification filters for treatment of saline wastewaters

This study investigated the performance and microbiome of cyclic denitrification filters (CDFs) for wood sulfur heterotrophic-autotrophic (WSHAD) saline wastewater. Wood-sulfur CDFs integrated into two pilot-scale marine recirculating aquaculture systems achieved high rates (103 ± 8.5 g N/(m3·d)). The combined use pine resulted in lower SO42? accumulation compared with prior wastewater studies alone. Although fish tank water quality parameters, including ammonia, nitrite, nitrate sulfide, were below inhibitory levels production, survival Poecilia sphenops observed studies. Heterotrophic was dominant removal mechanism during early operational stages, while autotrophic increased as readily biodegradable organic carbon released from chips decreased over time. 16S rRNA-based analysis CDF revealed that Sulfurimonas, Thioalbus, Defluviimonas, Ornatilinea notable genera contributed to performance.